- Pds1 / Esp1 Mutants
- pds1∆
- pds1∆ in nocodazole
- bub2∆ pds1∆ in nocodazole
- mad2∆ pds1∆ in nocodazole
- cdc20∆ pds1∆
- cdc20∆ pds1∆ clb5∆
- CLB5-db∆ pds1∆
- CLB5-db∆ pds1∆ cdc20∆
- CLB1 clb2∆ pds1∆
- esp1-ts
- GAL-PDS1-db∆ esp1-ts
- GAL-PDS1-db∆
- PDS1-db∆
- GAL-ESP1 cdc20-ts
GAL-PDS1-db∆
debug: ,
test user =
test db =
Simulation:
Change of parameters: kspds'=0.1, kd2pds"=kd3pds"=0, MDT=150.
Arrest: Exit mitosis at t=353.8 min with sister chromatids still attached.
Experiments:
Cohen-Fix, O., Peters, J.M., Kirschner, M.W. and Koshland, D. (1996). Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p. Genes Dev. 10:3081-3093.
[Abstract] [Article]
[Abstract] [Article]
Alexandru, G., Zachariae, W., Schleiffer, A. and Nasmyth, K. (1999). Sister chromatid separation and chromosome re-duplication are regulated by different mechanisms in response to spindle damage. EMBO J. 18:2707-2721.
[Abstract] [Article]
[Abstract] [Article]
Cohen-Fix, O. and Koshland, D. (1999). Pds1p of budding yeast has dual roles: inhibition of anaphase initiation and regulation of mitotic exit. Genes Dev. 13:1950-1959.
[Abstract] [Article]
[Abstract] [Article]
Tinker-Kulberg, R.L. and Morgan, D.O. (1999). Pds1 and Esp1 control both anaphase and mitotic exit in normal cells and after DNA damage. Genes Dev. 13:1936-1949.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Inviable. Cohen-Fix (1996) text, when mutant cells continuously overexpress Pds1-db∆ for > 6 hrs, some become aploid, they undergo cytokinesis in the absence of nuclear division.
Comments:
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